alp solution Search Results


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FUJIFILM alp substrate solutions
Alp Substrate Solutions, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FUJIFILM labassaytm alp, alkaline phosphatase
Labassaytm Alp, Alkaline Phosphatase, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioAssay Systems LLC alp solution
Alp Solution, supplied by BioAssay Systems LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Linaris GmbH horseradish peroxidase labelled secondary antibodies and alp or peroxidase substrate solutions vectastain® abc-ap staining kit
Horseradish Peroxidase Labelled Secondary Antibodies And Alp Or Peroxidase Substrate Solutions Vectastain® Abc Ap Staining Kit, supplied by Linaris GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing CWBio alp solution
SGMS1 facilitates osteogenic differentiation <t>of</t> <t>MSCs</t> (A and B) mRNA and protein expression levels of SGMS1 assessed via qPCR and western blot assays, respectively. (C and D) <t>ALP</t> and ARS staining of MSCs (100 μm; bar). (E) mRNA and protein (F) expression levels of OCN, Col-1, and OPN assessed via qPCR and western blot assays, respectively. Data are represented as mean ± standard deviation (SD). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.
Alp Solution, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alp+solution/alp+solution/pmc10966191-313-6-8
Average 90 stars, based on 1 article reviews
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Shanghai Hongqiao Pharmaceutical Co Ltd alp dye solution
SGMS1 facilitates osteogenic differentiation <t>of</t> <t>MSCs</t> (A and B) mRNA and protein expression levels of SGMS1 assessed via qPCR and western blot assays, respectively. (C and D) <t>ALP</t> and ARS staining of MSCs (100 μm; bar). (E) mRNA and protein (F) expression levels of OCN, Col-1, and OPN assessed via qPCR and western blot assays, respectively. Data are represented as mean ± standard deviation (SD). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.
Alp Dye Solution, supplied by Shanghai Hongqiao Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioDot Inc alp solution
SGMS1 facilitates osteogenic differentiation <t>of</t> <t>MSCs</t> (A and B) mRNA and protein expression levels of SGMS1 assessed via qPCR and western blot assays, respectively. (C and D) <t>ALP</t> and ARS staining of MSCs (100 μm; bar). (E) mRNA and protein (F) expression levels of OCN, Col-1, and OPN assessed via qPCR and western blot assays, respectively. Data are represented as mean ± standard deviation (SD). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.
Alp Solution, supplied by BioDot Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioAssay Systems LLC alp assay working solution
SGMS1 facilitates osteogenic differentiation <t>of</t> <t>MSCs</t> (A and B) mRNA and protein expression levels of SGMS1 assessed via qPCR and western blot assays, respectively. (C and D) <t>ALP</t> and ARS staining of MSCs (100 μm; bar). (E) mRNA and protein (F) expression levels of OCN, Col-1, and OPN assessed via qPCR and western blot assays, respectively. Data are represented as mean ± standard deviation (SD). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.
Alp Assay Working Solution, supplied by BioAssay Systems LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alp+solution/alp+assay+working+solution/pm23083929-88-21-35
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BioAssay Systems LLC alp substrate solution 4-npp elpn-500
SGMS1 facilitates osteogenic differentiation <t>of</t> <t>MSCs</t> (A and B) mRNA and protein expression levels of SGMS1 assessed via qPCR and western blot assays, respectively. (C and D) <t>ALP</t> and ARS staining of MSCs (100 μm; bar). (E) mRNA and protein (F) expression levels of OCN, Col-1, and OPN assessed via qPCR and western blot assays, respectively. Data are represented as mean ± standard deviation (SD). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.
Alp Substrate Solution 4 Npp Elpn 500, supplied by BioAssay Systems LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc avidin-conjugated alp and alp substrate, evan’s blue solution
SGMS1 facilitates osteogenic differentiation <t>of</t> <t>MSCs</t> (A and B) mRNA and protein expression levels of SGMS1 assessed via qPCR and western blot assays, respectively. (C and D) <t>ALP</t> and ARS staining of MSCs (100 μm; bar). (E) mRNA and protein (F) expression levels of OCN, Col-1, and OPN assessed via qPCR and western blot assays, respectively. Data are represented as mean ± standard deviation (SD). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.
Avidin Conjugated Alp And Alp Substrate, Evan’s Blue Solution, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FUJIFILM a premixed alp substrate solution
SGMS1 facilitates osteogenic differentiation <t>of</t> <t>MSCs</t> (A and B) mRNA and protein expression levels of SGMS1 assessed via qPCR and western blot assays, respectively. (C and D) <t>ALP</t> and ARS staining of MSCs (100 μm; bar). (E) mRNA and protein (F) expression levels of OCN, Col-1, and OPN assessed via qPCR and western blot assays, respectively. Data are represented as mean ± standard deviation (SD). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.
A Premixed Alp Substrate Solution, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing CWBio alp substrate solution
SGMS1 facilitates osteogenic differentiation <t>of</t> <t>MSCs</t> (A and B) mRNA and protein expression levels of SGMS1 assessed via qPCR and western blot assays, respectively. (C and D) <t>ALP</t> and ARS staining of MSCs (100 μm; bar). (E) mRNA and protein (F) expression levels of OCN, Col-1, and OPN assessed via qPCR and western blot assays, respectively. Data are represented as mean ± standard deviation (SD). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.
Alp Substrate Solution, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alp+solution/alp+substrate+solution/pm27696501-45-3-6
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SGMS1 facilitates osteogenic differentiation of MSCs (A and B) mRNA and protein expression levels of SGMS1 assessed via qPCR and western blot assays, respectively. (C and D) ALP and ARS staining of MSCs (100 μm; bar). (E) mRNA and protein (F) expression levels of OCN, Col-1, and OPN assessed via qPCR and western blot assays, respectively. Data are represented as mean ± standard deviation (SD). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.

Journal: iScience

Article Title: SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway

doi: 10.1016/j.isci.2024.109358

Figure Lengend Snippet: SGMS1 facilitates osteogenic differentiation of MSCs (A and B) mRNA and protein expression levels of SGMS1 assessed via qPCR and western blot assays, respectively. (C and D) ALP and ARS staining of MSCs (100 μm; bar). (E) mRNA and protein (F) expression levels of OCN, Col-1, and OPN assessed via qPCR and western blot assays, respectively. Data are represented as mean ± standard deviation (SD). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.

Article Snippet: Subsequently, the MSCs were incubated with ALP solution (CWBIO, Beijing, China) for 20 min.

Techniques: Expressing, Western Blot, Staining, Standard Deviation, Real-time Polymerase Chain Reaction

SGMS1 deficiency alters the MSC phenotype MSCs were cultured in OM for 14 days to induce osteogenic differentiation. (A and B) Differentiation was confirmed with ARS and ALP staining (100 μm; bar). (C and D) mRNA and protein expression levels of OCN, Col-1, and OPN determined via qPCR and western blot assays, respectively. (E) Immunofluorescence staining showing Runx2 expression (100 μm; bar). Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.

Journal: iScience

Article Title: SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway

doi: 10.1016/j.isci.2024.109358

Figure Lengend Snippet: SGMS1 deficiency alters the MSC phenotype MSCs were cultured in OM for 14 days to induce osteogenic differentiation. (A and B) Differentiation was confirmed with ARS and ALP staining (100 μm; bar). (C and D) mRNA and protein expression levels of OCN, Col-1, and OPN determined via qPCR and western blot assays, respectively. (E) Immunofluorescence staining showing Runx2 expression (100 μm; bar). Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.

Article Snippet: Subsequently, the MSCs were incubated with ALP solution (CWBIO, Beijing, China) for 20 min.

Techniques: Cell Culture, Staining, Expressing, Western Blot, Immunofluorescence, Real-time Polymerase Chain Reaction

Involvement of SGMS1 in the osteogenic differentiation of MSCs by regulating Cer/SM metabolism MSCs were cultured in OM for 14 days to induce osteogenic differentiation. (A and B) Cer and SM expression levels detected using LC-MS/MS. (C and D) Osteogenic differentiation verified via ARS and ALP staining (100 μm; bar). (E and F) mRNA and protein expression levels of OCN, Col-1, and OPN determined via qPCR and western blot assays, respectively. (G) Immunofluorescence staining to assess Runx2 expression (100 μm; bar). Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; Cer: ceramide; LC-MS/MS: liquid chromatography-tandem mass spectrometry; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; SM: sphingomyelin; NM: normal medium; OM: osteogenic medium.

Journal: iScience

Article Title: SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway

doi: 10.1016/j.isci.2024.109358

Figure Lengend Snippet: Involvement of SGMS1 in the osteogenic differentiation of MSCs by regulating Cer/SM metabolism MSCs were cultured in OM for 14 days to induce osteogenic differentiation. (A and B) Cer and SM expression levels detected using LC-MS/MS. (C and D) Osteogenic differentiation verified via ARS and ALP staining (100 μm; bar). (E and F) mRNA and protein expression levels of OCN, Col-1, and OPN determined via qPCR and western blot assays, respectively. (G) Immunofluorescence staining to assess Runx2 expression (100 μm; bar). Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; Cer: ceramide; LC-MS/MS: liquid chromatography-tandem mass spectrometry; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; SM: sphingomyelin; NM: normal medium; OM: osteogenic medium.

Article Snippet: Subsequently, the MSCs were incubated with ALP solution (CWBIO, Beijing, China) for 20 min.

Techniques: Cell Culture, Expressing, Liquid Chromatography with Mass Spectroscopy, Staining, Western Blot, Immunofluorescence, Liquid Chromatography, Mass Spectrometry, Real-time Polymerase Chain Reaction

PP2A was implicated in the regulation of SGMS1 in the osteogenic differentiation of MSCs MSCs were cultured in OM for 14 days to induce osteogenic differentiation. (A and B) Osteogenic differentiation of MSCs assessed via ALP and ARS staining (100 μm; bar). (C and D) mRNA and protein expression levels of OCN, Col-1, and OPN determined by via qPCR and western blot assays, respectively. (E) Immunofluorescence staining performed to assess Runx2 expression (100 μm; bar). Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium; PP2A: protein phosphatase 2A.

Journal: iScience

Article Title: SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway

doi: 10.1016/j.isci.2024.109358

Figure Lengend Snippet: PP2A was implicated in the regulation of SGMS1 in the osteogenic differentiation of MSCs MSCs were cultured in OM for 14 days to induce osteogenic differentiation. (A and B) Osteogenic differentiation of MSCs assessed via ALP and ARS staining (100 μm; bar). (C and D) mRNA and protein expression levels of OCN, Col-1, and OPN determined by via qPCR and western blot assays, respectively. (E) Immunofluorescence staining performed to assess Runx2 expression (100 μm; bar). Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium; PP2A: protein phosphatase 2A.

Article Snippet: Subsequently, the MSCs were incubated with ALP solution (CWBIO, Beijing, China) for 20 min.

Techniques: Cell Culture, Staining, Expressing, Western Blot, Immunofluorescence, Real-time Polymerase Chain Reaction