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Image Search Results
Journal: iScience
Article Title: SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway
doi: 10.1016/j.isci.2024.109358
Figure Lengend Snippet: SGMS1 facilitates osteogenic differentiation of MSCs (A and B) mRNA and protein expression levels of SGMS1 assessed via qPCR and western blot assays, respectively. (C and D) ALP and ARS staining of MSCs (100 μm; bar). (E) mRNA and protein (F) expression levels of OCN, Col-1, and OPN assessed via qPCR and western blot assays, respectively. Data are represented as mean ± standard deviation (SD). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.
Article Snippet: Subsequently, the MSCs were incubated with
Techniques: Expressing, Western Blot, Staining, Standard Deviation, Real-time Polymerase Chain Reaction
Journal: iScience
Article Title: SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway
doi: 10.1016/j.isci.2024.109358
Figure Lengend Snippet: SGMS1 deficiency alters the MSC phenotype MSCs were cultured in OM for 14 days to induce osteogenic differentiation. (A and B) Differentiation was confirmed with ARS and ALP staining (100 μm; bar). (C and D) mRNA and protein expression levels of OCN, Col-1, and OPN determined via qPCR and western blot assays, respectively. (E) Immunofluorescence staining showing Runx2 expression (100 μm; bar). Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium.
Article Snippet: Subsequently, the MSCs were incubated with
Techniques: Cell Culture, Staining, Expressing, Western Blot, Immunofluorescence, Real-time Polymerase Chain Reaction
Journal: iScience
Article Title: SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway
doi: 10.1016/j.isci.2024.109358
Figure Lengend Snippet: Involvement of SGMS1 in the osteogenic differentiation of MSCs by regulating Cer/SM metabolism MSCs were cultured in OM for 14 days to induce osteogenic differentiation. (A and B) Cer and SM expression levels detected using LC-MS/MS. (C and D) Osteogenic differentiation verified via ARS and ALP staining (100 μm; bar). (E and F) mRNA and protein expression levels of OCN, Col-1, and OPN determined via qPCR and western blot assays, respectively. (G) Immunofluorescence staining to assess Runx2 expression (100 μm; bar). Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; Cer: ceramide; LC-MS/MS: liquid chromatography-tandem mass spectrometry; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; SM: sphingomyelin; NM: normal medium; OM: osteogenic medium.
Article Snippet: Subsequently, the MSCs were incubated with
Techniques: Cell Culture, Expressing, Liquid Chromatography with Mass Spectroscopy, Staining, Western Blot, Immunofluorescence, Liquid Chromatography, Mass Spectrometry, Real-time Polymerase Chain Reaction
Journal: iScience
Article Title: SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway
doi: 10.1016/j.isci.2024.109358
Figure Lengend Snippet: PP2A was implicated in the regulation of SGMS1 in the osteogenic differentiation of MSCs MSCs were cultured in OM for 14 days to induce osteogenic differentiation. (A and B) Osteogenic differentiation of MSCs assessed via ALP and ARS staining (100 μm; bar). (C and D) mRNA and protein expression levels of OCN, Col-1, and OPN determined by via qPCR and western blot assays, respectively. (E) Immunofluorescence staining performed to assess Runx2 expression (100 μm; bar). Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ALP: alkaline phosphatase; ARS: Alizarin red S; MSC: mesenchymal stem cell; OCN: osteocalcin; OPN: osteopontin; qPCR: quantitative polymerase chain reaction; SGMS1: sphingomyelin synthase 1; NM: normal medium; OM: osteogenic medium; PP2A: protein phosphatase 2A.
Article Snippet: Subsequently, the MSCs were incubated with
Techniques: Cell Culture, Staining, Expressing, Western Blot, Immunofluorescence, Real-time Polymerase Chain Reaction